Tonicity induced changes in volume and refractive index of suspended cells quantified with digital holographic microscopy


  • Björn Kemper University of Muenster, Germany
  • Jens Klokkers University of Muenster, Germany
  • Sabine Przibilla University of Muenster, Germany
  • Angelika Vollmer University of Muenster, Germany
  • Steffi Ketelhut University of Muenster, Germany
  • Gert von Bally University of Muenster, Germany
  • Hermann-Joseph Pavenstädt University of Muenster, Germany
  • Eberhard Schlatter University of Muenster, Germany
  • Bayram Edemir



The analysis of changes of the volume and the intracellular solute concentrations of biological cells represents an important topic in cytometry. We present results from a comparative study in which the cell volume and the refractive index of living rat inner medullary collecting duct cells and human pancreatic tumor cells was determined in media with different tonicity by using quantitative digital holographic phase microscopy. The results show that the inverse cell volume correlates linearly with the integral cellular refractive index which is directly related to the concentration of intracellular solutes.

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How to Cite

B. Kemper, “Tonicity induced changes in volume and refractive index of suspended cells quantified with digital holographic microscopy”, Photonics Lett. Pol., vol. 4, no. 2, pp. pp. 45–47, Jun. 2012.