Characterizing electrokinetic mobility of microparticles using in-line holographic microscopy
AbstractTraditionally, electrokinetic phenomena are demonstrated under a microscope. Since an ordinary light microscope has a short depth of focus and in many cases the electrokinetic phenomenon may take place in a relatively large volume, it is essential to use a microscopic method which has a long depth of focus and adequate depth resolution. The advantage of using a transparent platform and in-line holographic imaging for particle tracking is shown. Dielectrophoretic (DEP) particle motion is demonstrated on a fully transparent platform with 2D indium-tin-oxide (ITO) electrodes, and images of the whole sample volume are reconstructed using the digital in-line holographic method (DIHM) in order to find the 3D positions and speeds of the particles influenced by the DEP force and other electrokinetic forces. DIHM imaging proved to be a practical tool for analyzing particle motion due to multiple, simultaneously acting, electrokinetic forces.
Full text: PDF
- H. Watarai, T. Sakamoto, S. Tsukahara, "In Situ Measurement of Dielectrophoretic Mobility of Single Polystyrene Microparticles", Langmuir 13, p.2417-2420 (1997). [CrossRef]
- H. Morgan, N. G. Green, AC electrokinetics: Colloids and nanoparticles (England, Research Studies Press LTD 2003).
- M. Kuokkanen, Cent. Eur. J. Phys (2009).
- S. K. Jericho, P. Klages, J. Nadeau, E. M. Dumas, M. H. Jericho, H. J. Kreuzer, "In-line digital holographic microscopy for terrestrial and exobiological research", Planetary and Space Science, 58, p.701-705, (2010). [CrossRef]
- J. Garcia-Sucerquia, W. Xu, S. K. Jericho, M. H. Jericho, H. J. Kreuzer, "4-D imaging of fluid flow with digital in-line holographic microscopy", Optik, 119, p.419-423.(2008). [CrossRef]
- R. Pethig, "Preface to Special Topic: Dielectrophoresis", Biomicrofluidics, vol. 4, (2010). [CrossRef]
- M. P. Hughes, Nanoelectromechanics in Engineering and Biology (CRC Press 2003). [DirectLink]
- T. B. Jones, Electromechanics of Particles (Cambridge University Press 1995). [CrossRef]
- H.A. Pohl, "The Motion and Precipitation of Suspensoids in Divergent Electric Fields", J. Appl. Phys. vol. 22, no. 7, pp. 869-871, (1951). [CrossRef]
How to Cite
Haapalainen, M., Kaikkonen, V., Kinnunen, I., & Mäkynen, A. (2011). Characterizing electrokinetic mobility of microparticles using in-line holographic microscopy. Photonics Letters of Poland, 3(2), pp. 85–87. https://doi.org/10.4302/photon. lett. pl.v3i2.221